Question 1

What is offered in each of the service stages?

Accepted Answer

The deliverables are clear and defined together with the customer before the start of the service. As a guideline we provide the following information after each work package:

1) In silico and focused screening: 
- Samples of up to 100 mg for the best 3-5 candidates. 
- General immobilization characteristics (material and chemistry). The full details of the immobilization are not shared until WP2.
- Report with details on the bioinformatic and screening results.

2) Optimization: 
- Samples of up to 5g for the best 1-3 samples.
- Immobilization protocol.
- Report with full characterization of the preparations in terms of activity, stability and reusability.

3) Scale up:
- Samples of up to 250 g of the best preparation. 
- SOP and scalability metrics.
- Final report.

Question 2

Can I request a feasibility test before full-scale immobilization?

Accepted Answer

Yes, that's exactly what the first work package of our service is about - ensuring we have significant hits before committing to a longer project. In the first stage, in only 4 weeks, the bioinformatics and focused screening allow us to find significant hits in terms of activity, stability and reusability.

Question 3

Do you offer the bioinformatic analysis only?

Accepted Answer

Yes, we can perform the bioinformatics only and give you recommendations on the immobilization techniques that best suit your protein. Our database is propietary, and therefore cannot be shared. 
Keep in mind though, that even in our own development, we take advice from the model but lab validation is always the best way to go!

Question 4

How long does it take for a full service?

Accepted Answer

We strive to be as fast as possible - and that is why the first part of the service, the focused screening is done in only 4 weeks. A full service, where we optimize the immobilization protocol to your reaction or protein of interest, typically takes from 14 weeks.

Question 5

How is intellectual property (IP) managed?

Accepted Answer

We ensure clarity from the start. All background IP belongs to and remains with you. The created immobilization technology can be in-licensed after service completion, or we can produce the immobilized enzyme for you. We adapt to what works best for you. And of course, we sign an NDA before starting any project!

Question 6

Do you work with crude lysate or only purified enzymes?

Accepted Answer

We work with what you have. We have experience with lysates, semi-purified enzymes and purified enzymes.

Question 7

What supports or chemistries do you use for immobilization?

Accepted Answer

We have a broad range of supports. Different materials with different physicochemical properties and sizes. All to ensure the maximum flexibility and to make sure we can tailor each immobilization to your desired enzyme.

Frequently asked questions