🧬 Advanced Immobilization via Bioinformatics (CapiPy)
CapiPy was the cornerstone of our journey. By integrating bioinformatics specifically adapted for enzyme immobilization, we introduced structure and predictability into a field traditionally governed by trial and error. Immobilization decisions can now be driven by data and protein-specific insights, saving time, resources, and frustration.
🛑 The Problem: Protein immobilization remains a trial-and-error process, making it inefficient and time-consuming.
🔄 Chemo‑Enzymatic Flow Synthesis of Chiral α‑Aryl Amines
Seamless chemo‑enzymatic flow workflows deliver high-purity products with waste-minimized processing. Chiral α-aryl amines are gaining momentum as essential building blocks for advanced pharmaceuticals. Our integrated system demonstrates how flow chemistry and enzymatic steps can be harmonized for continuous production of high-value targets.
🛑 The Problem: Chiral α-heteroaryl amines are critical pharmaceutical precursors but traditional routes are step‑intensive and low-yield.
🧩 Multi-Enzyme System Optimization via High‑Throughput Screening
We collaborated with Prof. Fernando Gallego from biomaGUNE to immobilize multiple enzymes simultaneously, a task that is challenging due to the varied requirements of each protein. Our support from the bioinformatics offered rational explanations for the immobilization results, enabling a scalable solution to rationally select carriers and chemistries based on comparative performance, rather than trial alone.
🛑 The Problem: Designing immobilization strategies for cascades of multiple enzymes is complex and inefficient using individual trial-and-error.
♻️ Continuous Flow IRED Immobilization for Heterocyclic Amines
Immobilized IREDs proved transformative—enabling efficient, selective, and scalable synthesis of bio-relevant heterocycles. These structures are prevalent in pharmaceuticals, pesticides, and flavors, making their efficient synthesis both economically and strategically important. We used our full stack: bioinformatics, enzyme immobilization, and flow chemistry.
🛑 The Problem: Immobilization of IREDs in an efficient and reusable manner proved difficult due to intrinsic properties of these enzymes.